Effect of Kif15 on the proliferation,cell cycle and apoptosis of astrocytes in rat

Objective:To investigate the effects of Kif15 on the proliferation,cell cycle and apoptosis of astrocytes in rat,and its mechanism.Methods:Astrocytes was isolated from one day old rat,the NC-siRNA and Kif15-siRNA were transfected into the cells,and the cells without any treatment was set as the control group.The expression levels of Kif15 protein in each group were detected using Western blotting after 48 h of transfection.The cell proliferation,cell cycle and apoptosis,and protein expression levels of PCNA,cyclinD1,Cleaved caspase3,p38 and p-p38 were detected using CCK8 assay,flow cytometry and Western blotting,respectively.Results:The expression level of Kif15 protein could be significantly inhibited in the cells transfected with Kif15-siRNA.Compared with the control group and NC-siRNA group,the cell survival rate,G₂/M cells,and expression levels of PCNA,cyclinD1 and p-p38 proteins significantly decreased,the cell apoptosis rate,G₁ cells and expression level of cleaved Caspase 3 protein significantly increased in Kif15-siRNA group(P<0.01),and the difference of the expression level of p38 between three groups was not statistically significant(P>0.05).Conclusions:The inhibiting of Kif15 in astrocytes can significantly reduce the proliferation of cells,block the cell in G₁ phase and promote apoptosis,the mechanism of which is related to the inhibition of p38 signaling pathway.