Effect of the silencing p62 gene expression by RNA interference on proliferation of cervical cancer HeLa cells
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Abstract
Objective:To investigate the effects of downregulating the expression of p62 gene by shNA transfection on proliferation of cervical cancer HeLa cells.Methods:The lentiviral vector containing shRNA sequence of silencing p62 gene was constructed,and transfected into the cervical HeLa cells(shRNA group).The negative control group(shNC group) was set,and the non-lentiviral vector was set as blank control group.The expression levels of p62 mRNA,cell proliferation and cell colony formation rate in 3 groups were detected using qRT-PCR,CCK-8 assay and colony formation assay,respectively.Results:The lentiviral vector containing shRNA sequence of silencing p62 gene was successfully constructed.The expression of p62-mRNA in shRNA group and shNC group relative to the blank control group were (0.18±0.08) and (1.01±0.12),respectively.The interfere rate of shRNA was 81.00%.Compared with the negative control group and blank control group,the cell proliferation and colony formation rates in shRNA group were slow and little(P<0.05 and P<0.01),respectively.Conclusions:Silencing the expression of p62 gene can reduce the proliferation of cervical cancer HeLa cells.The p62 gene may play an important role in the proliferation of cervical cancer HeLa cells.
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