Protective effect of erythropoietin on the lung of rats after cardiopulmonary resuscitation

Objective:To evaluate the protective effects of erythropoietin(EPO) on the lung of rats after cardiopulmonary resuscitation(CPR).Methods:The rats were randomly divided into the sham group,CPR group and EPO group.The levels of MDA,SOD and GSH-Px in lung were detected using ELISA.Results:At the time of restoration of spontaneous circulation,the levels of CaO₂ in CPR group and EPO group were lower than that in sham group(P<0.05),and with the lengthening of restoration time,the level of which gradually returned to normal.After 3 h,6 h and 12 h of CPR,the levels of MDA in CPR group and EPO group were significantly higher than that in sham group(P<0.01),the levels of MDA in CPR group after 6 h and 12 h of CPR were significantly higher than that after 3 h of CPR(P<0.01),and the levels of MDA in EPO group after 6 h and 12 h of CPR were lower than that in CPR group(P<0.05 and P<0.01).Compared with the sham group,the levels of SOD and GSH-Px in CPR group and EPO group decreased after 3 h,6 h and 12 h of CPR(P<0.05 to P<0.01),the levels of SOD and GSH-Px in CPR group after 6 h of CPR were significantly lower than those after 3 h of CPR(P<0.01),and the levels of SOD and GSH-Px in CPR group after 12 h of CPR were significantly higher than those after 6 h of CPR(P<0.01).The levels of SOD and GSH-Px in EPO group after 3 h,6 h and 12 h of CPR were significantly lower than those in CPR group(P<0.01).Before treatment with EPO,the content of MDA was negatively correlated with SOD(P<0.05) and GSH-Px(P<0.01) in rats.After treatment with EPO,the content of MDA was negatively correlated with GSH-Px(P<0.05),but uncorrelated with SOD in rats(P>0.05).Conclusions:EPO can reduce the oxidative stress and injury of lung,and promote the recovery of lung function after CPR.