Expression of miRNA-27b in placenta of preeclampsia patients, and the effect of Salvia miltiorrhiza on its expression

Objective: To investigate the expression of miRNA-27b in placenta of preeclampsia patients,and the effects of Salvia miltiorrhiza on its expression.Methods: Twenty placental tissue samples from severe preeclampsia patients,20 placental tissue samples from mild preeclampsia and 20 placental tissue samples from healthy placental tissue were divided into the severe preeclampsia group,mild preeclampsia group and control group,respectively.The expression levels of miRNA-27b and leptin mRNA were detected using the real-time fluorescence quantitative PCR(qRT-PCR).The miRNA-27b was processed using transient transfection,and the leptin mRNA level and human sertoli cells HTR-8 apoptosis were detected using the qRT-PCR and flow cytometry,respectively.Results: The expression levels of miRNA-27b and leptin mRNA in severe preeclampsia group were higher than those in mild preeclampsia group and control group,and the expression levels of miRNA-27b and leptin mRNA in mild preeclampsia were higher than those in control group(P<0.05).The expression levels of miRNA-27b and leptin mRNA in miRNA-27b mimics group significantly increased compared with the Con group and NC group(P<0.05),and the expression level of miRNA-27b in miRNA-27b inhibitor group decreased compared with the Con group and NC group(P<0.05).The differences of the expression levels of miRNA-27b and leptin mRNA between Con group and NC group were not statistically significant(P>0.05).The apoptosis levels of HTR-8 cells in miRNA-27b mimics group and miRNA-27b inhibitor group were higher and lower than that in Con group and NC group,respectively(P<0.05).The expression levels of miRNA-27b and leptin mRNA in severe preeclampsia group and mild preeclampsia group decreased compared with before Salvia miltiorrhiza treatment(P<0.05).Conclusions: The expression levels of miRNA-27b and leptin mRNA in preeclampsia placenta increase,miRNA-27b can promote the apoptosis of sertoli cells.miRNA-27b plays a positive regulation to leptin,both may work together in the development of preeclampsia.Salvia miltiorrhiza can inhibit the expression of miRNA-27b and leptin in preeclampsia.