Effect of compound Kushen injection on the pharmacokinetics of docetaxol in rats

Objective:To develop a high performance liquid chromatography(HPLC) method for the determination of docetaxol concentration,and investigate the effects of different dosages of compound Kushen injection on the pharmacokinetics of multiple-dose docetaxol in rats.Methods:Twenty-four SD rats were randomly divided into the high-dose,medium-dose,low-dose and control groups(6 rats each group).The high-dose,medium-dose,low-dose and control groups were injected with 2 times clinical equivalent compound Kushen injection(3.6 mL/kg),clinical equivalent compound Kushen injection(1.8 mL/kg),1/2 clinical equivalent compound Kushen injection(0.9 mL/kg) and 0.9% normal saline solution by caudal vein for 7 days,respectively,and all rats were injected with 15 mg/kg of docetaxol after 5 min of administration on the seventh day.The blood samples in four groups at different time-points were analyzed,and the pharmacokinetic parameters were calculated.Results:After multiple-dose,the AUC and C_max in high-dose and medium-dose groups were lower than those in control group(P<0.05),and the difference of which between low-dose group and control group was not statistically significant(P>0.05).Conclusions:The compound Kushen injection can reduce the blood concentration of docetaxol,there is a dose-dependent relationship between compound Kushen injection and docetaxel,which can provide a reference in clinical medication.