Diagnostic value of DNA quantitative analysis in early screening of cervical lesion, and its clinical significance in cytology of ASCUS

Objective:To assess the diagnostic value of DNA quantitative analysis in early screening of cervical lesions,and investigate its clinical significance in cytology of atypical squamous cells of undetermined significance(ASCUS).Methods:The thinprep cytology test(TCT) and cell DNA quantitative analysis were implemented in 1 980 cervical census from January 2015 and December 2016.The biopsies under colposcopy in 241 cases were detected.Under the histological diagnosis being set as the gold standard,the diagnostic value of DNA quantitative analysis and its clinical significance in cytology of ASCUS were evaluated.Results:Among 241 cases,7 cases in normal DNA ploidy group(128 cases),7 cases in heteroploid cell group(54 cases) and 30 cases in equal to or more than 3 heteroploid cell group(59 cases) were diagnosed for cervical intraepithelial neoplasia grade 2(CIN Ⅱ),respectively.The results of DNA ploidy analysis and histopathology showed that the lesion grade was positively correlated with the abnormal DNA ploidy number(r_s=0.439,P<0.01),the sensibility and specificity of which were 84.1% and 61.4%,respectively.Among 57 cases with ASCUS,1 to 2 abnormal DNA ploidy cells in 8 cases, ≥ 3 abnormal DNA ploidy cells in 28 cases and normal DNA ploidy cell in 21 cases were identified.The cases of grade CIN Ⅱ and above in three groups were 1 case,13 cases and 1 case,respectively.The histopathology grade in ASCUS patients complicated with abnormal DNA ploidy was positively correlated with the abnormal DNA ploidy(r_s=0.424,P<0.01).Conclusions:DNA quantitative detection can improve the early screening rate of the cervical lesions,the risk of CIN Ⅱ or above in ASCUS patients complicated with heteroploid cell is the highest.The combination of DNA quantitative analysis and cytology can improve the screening quality to guide the clinical treatment.