The mechanism of oxaliplatin inhibits proliferation of hepatocellular carcinoma cell through p53 signaling pathway

Objective:To investigate the effects of oxaliplatin(L-OHP) on the proliferation and apoptosis of mutant type hepatocellular carcinoma cell line HuH-7 and wild type hepatocellular carcinoma cell line HepG2,and the role of p53 signaling pathway-related protein.Methods:The inhibiting effects of L-OHP on the proliferation of HuH-7 and HepG2 cells were detected using MTT assay.After HuH-7 and HepG2 cells were treated with L-OHP,the morphology changes of two cell lines were observed by inverted microscope,the cell cycle distribution was measured by flow cytometry,and the expression of p53 signaling pathway-related protein was detected using Western blot.Results:L-OHP could inhibit the proliferation of HuH-7 and HepG2 cells,which showed the concentration and time-dependent manner(P<0.01).After treatment with L-OHP,the cells became round,off the wall and apoptosis.L-OHP could induce the HuH-7 and HepG2 cells stasising at S phase of cell cycle,and the proportion of cells increased with the increasing the concentration of L-OHP(P<0.01).L-OHP could activate the p53 signaling pathway,and regulate the protein expression levels of p21,Bax,Bcl-2 and p53 in HuH-7 and HepG2 cells(P<0.05),but the expression change of p-p53 in HuH-7 cells was not obvious.Conclusions:L-OHP can induce the apoptosis of hepatocellular carcinoma cells by activating the p53 signaling pathway,the mechanism of which may be related to regulating the expression of p53,p-p53,p21,Bax and Bcl-2 in p53 signaling pathway.