Effect of arsenic trioxide on the proliferation and gene expression of APC in bladder cancer T24 cells

Objective:To observe the effects of arsenic trioxide(As₂O₃) on the proliferation and gene expression of adenomatous polyposis coli(APC) in human bladder cancer T24 cells.Methods:The T24 cells were processed with 2,4 and 8μmol/L of As₂O₃,the proliferation inhibition rate was detected using MTT assay,the apoptosis was detected using the deoxyribose situ terminal transferase labeling,and the expression levels of APC mRNA and protein were detected using RT-PCR and Western blot,respectively.Results:The 2 to 8 μmol/L of As₂O₃ treated T24 cells for 72 h could effectively inhibit the cell proliferation(P<0.01),promote the cell apoptosis(P<0.01),and increase the expression of APC mRNA and protein(P<0.01).Conclusions:As₂O₃ (2~8μmol/L) can effectively inhibit the proliferation,and induce the apoptosis of T24 cells,the mechanism of which may be related to the upregulation of APC gene expression.