Effect of TRPV4 in the diastole of thoracic aorta in hypertensive mice

Objective:To investigate the role of transient receptor potential vanilloid type 4(TRPV4) in the diastole of thoracic aorta in hypertensive mice,and explore its mechanism.Methods:The vascular tension of thoracic aorta in mouse was measured.The vasoconstriction was pre-contracted with 1 μmol/L phenylephrine hydrochloride(Phe).The vascular tension changes mediated by ACh and GSK1016790A in control group and TRPV4 inhibitor HC067064 treating group were observed,and the vascular tension changes mediated by ACh and GSK1016790A in wild type and TRPV4 knockout(TRPV4-/-) mice were observed.The vascular tension of the thoracic aorta rings in normal diet group and high salt diet group were measured to detect the vascular tension change induced by GSK1016970A.Results:The arterial diastole response induced by ACh and GSK1016790A in HC067064 group was significantly lower than that in control group(P<0.05).The arterial diastole response induced by ACh and GSK1016790A in thoracic aorta rings of TRPV4-/-mice was lower than that in wildtype mice(P<0.05).The arterial diastole response induced by GSK1016790A in high salt diet group was lower than that in normal diet group(P<0.05).Conclusions:TRPV4 is involved in the diastole of the thoracic aorta in mice.The action mechanism is to stimulate the opening of the TRPV4 channel,promote the extracellular calcium ion influx into the intracellular,and relax the blood vessels after a series of signal transduction.But for the pathological models,such as highsalt diet,the relaxation of TRPV4 in thoracic aorta can be inhibited.