Effect of sodium acetate ringer's solution combined with ulinastatin on the expression of NF-κB p65 protein and cytokines in liver tissue of hemorrhagic shock rats

Objective:To investigate the effects of sodium acetate ringer's solution combined with ulinastatin on the expression of NF-κB p65 protein and cytokines in liver tissue of hemorrhagic shock rats.Methods:Twenty-four SD rats were randomly divided into the hemorrhagic shock without resuscitation group(CR group),acetate ringer's solution group(AR group) and sodium acetate ringer's solution combined with ulinastatin group(WA group)(8 rats each group).A rat model of hemorrhagic shock was established.The expression levels of TNF-α mRNA,IL-4 mRNA and IL-10 mRNA in liver tissue were detected using RT-PCR.The relative expression levels of p65(Ser536) phosphorylation and p65(Lys310) acetylated protein in liver tissue were detected by Western Blot.The liver tissue morphology in three groups were observed under light microscope.Results:Liver histopathology results showed that the degree of liver tissue damage in WA group was lighter than that in CR group and AR group.In liver tissue,compared with CR group and AR group,the TNF-α mRNA expression decreased in WA group(P<0.01 and P<0.05),the IL-4 mRNA expression increased(P<0.01 and P<0.01) and the IL-10 mRNA expression increased(P<0.01 and P<0.05).Compared with CR group and AR group,the expression level of p65(Ser536) phosphorylated protein decreased in WA group(P<0.05).Compared with CR group and AR group,the expression level of p65(Lys310) acetylated protein decreased in WA group(P<0.05).Conclusions:Sodium acetate ringer's solution combined with ulinastatin alleviates liver injury in hemorrhagic shock rats,which may be through reducing NF-κB p65 protein activation,reducing pro-inflammatory factor TNF-α level,and enhancing anti-inflammatory factors IL-4 and IL-10 level.