The establishment of metabolic syndrome rat model induced by fructose and high-fat diet

Objective:To establish the rat model of metabolic syndrome(MS) with abnormal glucose and lipid metabolism,and provide a basis for studying the pathogenesis of metabolic syndrome,and developing dietary treatment measures.Methods:Thirty SD male rats with(210±10)g were randomly divided into the observation group and control group.The observation group were fed with 15% fructose water mixed with high-fat diet,and the control group were fed with regular diet for 10 weeks,respectively.The levels of body weight,body length,waist circumference,fasting blood glucose,serum triglycerides,high-density lipoprotein cholesterol and insulin in two groups were detected.Results:The levels of body weight,Lee's index,fasting blood glucose,and serum triglycerides,high-density lipoprotein cholesterol in observation group were higher than those in control group(P<0.01),and the level of HDL-C in observation group was lower than that in control group after 10 weeks of test(P<0.01).After 4,8 and 10 weeks of test,the waist circumference in observation group was more than that in control group(P<0.01 to P< 0.05),the fasting insulin and steady-state insulin resistance index in observation group were higher than those in control group(P<0.01 to P<0.05).Twelve rats arrived at central obesity criteria,11 rats arrived at the high TG criteria,11 rats arrived at low HDL-C critia,and 12 rats with high blood glucose were found.Combined with the above indicators,two or three indexes of 4 SD rats failed to meet the criteria,and the rat model of metabolic syndrome rate was 73.3%.Conclusions:The 15% fructose water combined with high-fat diet can induce the rat model of metabolic syndrome in a short time.