Effect of virus macrophage inflammatory protein Ⅱ N-terminal peptide on the expression spectrum of miRNAs in breast cancer

Objective: To investigate the effects of virus macrophage inflammatory protein-Ⅱ(vMIP-Ⅱ) N-terminal 21 amino acids peptide(NT21MP) on the expression profiles of miRNAs in breast cancer MCF-7 cell.Methods: The RNA in each group cells was extracted,and transcribed into cDNA.The experiment was designed according to the 2^-△△Ct analytical method,and the miRNA expression profile was analyzed using the quantitative analysis of SYBR Green I.Results: A total of 168 miRNAs genes were detected,and the genes of expression upregulating two-fold or downregulating 0.5-fold were screened.Compared with the S group,the expression levels of miR-223-3p,miR-451a,miR-128,miR-489,miR-141-3p,miR-320a,miR-155-3p,miR-335-3p and miR-320e increased,and the expression levels of miR-15a-5p and miR-339-5p decreased in N group.Conclusions: The differential expression profiles of miRNAs regulated by NT21MP were screened to provide a basis for studyiny the mechanism of miRNAs in th regulation of NT21MP.