Effect of over-expression of Periostin protein on the biological function of endothelial progenitor cells

Objective To construct the over-expression of Periostin protein lentiviral vector, and investigate its effects on the biological function of endothelial progenitor cells(EPCs).

Methods The mouse EPCs were isolated from bone marrow, cultured and identified.The LV5/Periostin lentiviral vector was constructed, and transferred into the EPCs.The expression of green fluorescent protein(GFP) in transfected cells was observed under fluorescence microscope.The expression level of Periostin in EPCs was verified by Western blotting, the proliferation and apoptosis of infected cells were detected using the cell counting kit-8(CCK-8) assay and flow cytometry, and the migration ability and invasion ability of cells were detected using the scratch and Transwell assgy.

Results The expression of GFP in EPCs was obvious.Compared with the control group, the proliferation activity of cells was lower, and the number of apoptosis cells were more in lentiviral vector group(P < 0.05).Compared with the control group, the migration rate of cells was higher, the number of invasion cells were more in lentiviral vector group(P < 0.05).

Conclusions EPCs can be isolated from bone marrow, and cultured and identified.The over-expression lentiviral vector of Periostin protein can reduce the growth and proliferation, promote the apotosis, and enhance the migration and invasion ability of EPCs.