Effect of anisodamine pretreatment on the lung injury induced by hepatic ischemia-reperfusion in rats

Objective To investigate the effects of anisodamine pretreatment on the lung injury induced by hepatic ischemia-reperfusion in rats.

Methods Forty-eight healthy male Sprague-Dawley rats were randomly divided into the sham operation group(group S), hepatic ischemia-reperfusion group(group IR) and anisodamine group(group A) using a random number table(16 rats each group).The hepatic portal in group S was dissected, and the ischemia-reperfusion model of 70% liver in group IR and group A were established.The group A was injected with 2 mg/kg anisodamine before 30 min of blocking liver blood flow and the group S and group IR were injected with the same volume of 0.9% sodium chloride solution.The rats were sacrificed after 3 h of reperfusion, the lung tissue was harvested, stained using HE, and the result of which was observed under light microscope.The ratio of wet to dry(W/D) of lung weight was calculated.The levels of malondialdehyde(MDA), superoxide dismutase(SOD) and myeloperoxidase(MPO) in lung tissue were detected using thiobaituricacid, xanthinoxidase and Hydrogen oxide reduction method, respectively.The expression levels of heme oxygenase-1(HO-1) and inducible nitric oxide synthase(iNOS) in lung tissue were determined by immunohistochemistry.

Results The Results of HE staining in lung tissue showed that the lung injury in IR and A groups were more serious than that in group S, and the lung injury in group A was lighter than that in group IR.Compared with the group S, the ratio of W/D, MDA content, MPO activity and HO-1 and iNOS protein expression level significantly increased(P < 0.01), and the SOD activity significantly decreased in IR and A groups(P < 0.01).Compared with the group IR, the ratio of W/D, MDA content, MPO activity and iNOS protein expression level significantly decreased(P < 0.01), and the HO-1 protein level and SOD activity in lung tissue significantly increased in group A(P < 0.01).

Conclusions Anisodamine pretreatment can alleviate the lung injury induced by hepatic ischemia-reperfusion in rats.