ObjectiveTo optimize the extraction technology of bishonokiol A from the Magnolia grandiflora seeds.
MethodsThe concentration of bishonokiol A in different extracting solvents was determined using RP-HPLC method.The yield rate of bishonokiol A was set as the indictor, the effects of the ratio of material-to-liquid, soaking duration, and extraction times on the extraction technology were observed using L9(34) orthogonal test.
ResultsThe orthogonal test showed that the ratio of material-to-liquid for 130, soaking duration for 48 h, and extraction times for three times were the best technological condition of bishonokiol A extraction.
ConclusionsThe optimized extraction process designed by orthogonal test is simple, stable and reliable.