The detection rate and genotype analysis of Mycoplasma pneumonie in bronchoalveolar lavage fluid

ObjectiveTo detect the distribution of Mycoplasma pneumonie(MP) in bronchoalveolar lavage fluid of 136 children, and preliminarily understand the genotype of MP in Hefei Anhui province.

MethodsThe MP infection cases in 136 bronchoalveolar lavage fluid samples were screened using PCR.The genotype of MP was analyzed using nest multiplex PCR, and the DNA temple of FH-MP standard strain was set as control.The recombinant plasmid was constructed using the amplified DNA fragment cloned by nest multiplex PCR, and sequenced.

ResultsFifty-two positive MP samples were screened in 136 cases of bronchoalveolar lavage fluid using PCR.The nest multiplex PCR results showed that the genotype of MP positive samples was type P1-Ⅰ, and the genotype of standard strain FH-MP was type PCP1-Ⅱ.The sequencing results of the recombinant plasmid showed that the cloned fragment was right.

ConclusionsThe genotype of epidemic strains of MP is type P1-Ⅰ, and the positive rate of MP infection is 38.2% in Hefei.