Study on the mechanism of the tumor vascular endothelial cells influencing the migration ability of glioma cells

ObjectiveTo investigate the role of tumor vascular endothelial cells (GVEC) in the migration of glioma tumor cells.

MethodsThe vascular endothelial cells (VEC) and GVEC were isolated, and co-cultured with glioma cell line CGH5 in vitro, respectively.The CHG5 cells were set as the control.The effects of GVEC on the migration ability and cytoskeleton of CHG5 cells were analyzed using the Transwell migration assay and immunofluorescence staining, respectively.The expression levels of Rac1 and Cdc42 in CHG5 cells after co-culture were detected using Western blotting.

ResultsThe migration ability of CHG5 co-cultured with GVEC was significantly higher than that in CHG5 co-cultured with VEC and CHG5 culture alone (P < 0.05).The results of cytoskeleton immunofluorescence staining showed that the morphology of tubulin in CHG5 cells changed, and the tubulin was thick, circular and radial arrangement after the CHG5 cells were co-cultured with GVEC.The results of Western blotting showed that the expression levels of Rac1 and Cdc42 in CHG5 cells significantly increased after the CHG5 cells were co-cultured with GVEC (P < 0.05), while no obvious change of the expression levels of Rac1 and Cdc42 in CHG5 cells was found after the CHG5 cells were co-cultured with VEC (P>0.05).

ConclusionsThe role of tumor cell vascularity is crucial in the migration of glioma cells.The GVEC can promote the migration of tumor cells by changing the cellular tubulin structure, and up-regulating the expression levels of Rac1 and Cdc42 proteins.