Study on the model of lung injury induced by sepsis in mice

ObjectiveTo explore the related mechanism of the lung injury of in the mouse model of sepsis constructed by cecal ligation and puncture.

MethodsA total of 42 healthy male C57BL/6 mice were randomly divided into the control group, sham operation group and sepsis group(14 mice each group).The control group was not treated, the sham group was treated with cecal ligation, and the sepsis group was treated with cecal ligation combined with puncture.The lung tissue in three groups was collected after 24 hours of treatment, and the MSS score, survival rate, morphological change of lung tissue, score of lung injury, number of neutrophils and expression levels of tumor necrosis factor α(TNF-α) and interleukin-6(IL-6) in lung tissues were analyzed in three groups.

ResultsThe MSS score, score of lung injury, number of neutrophils and expression levels of TNF-α and IL-6 in sepsis group were higher than those in control group and sham operation group(P < 0.01).

ConclusionsSepsis can lead to the acute lung injury and increasing expression level of inflammatory factors, and decrease the survival rate of mice.The related indicator of reflecting lung tissue injury change can reflect the severity of sepsis, and study its related mechanism.