Effect and mechanism of salvianolic acid B on cerebral ischemia in rats

ObjectiveTo investigate the effect of salvianolic acid B (Sal B) against cerebral ischemia in rats.

MethodsThe middle cerebral artery occlusion(MCAO) was established in 50 rats to observe the effects of Sal B at different dosage on ischemic stroke.The rats were divided into 5 groups:blank control(1 mL/kg 0.9% NaCl solution), positive control(10 mg/kg aspirin), high dosage SalB(40 mg/kg Sal B), medium dosage SalB(20 mg/kg Sal B) and low dosage SalB(10 mg/kg Sal B)groups, And each chemical and physical data of cerebral ischemia was recorded for further analysis.

ResultsThere were significant differences in cerebral infarction area and behavioral score among each group(P < 0.01 and P < 0.05).Compared with the blank control group, Sal B significantly reduced cerebral infarction area with increasing doses(P < 0.01).There were significant differences in thrombus weight and platelet aggregation among the five groups(P < 0.01).The thrombus weight in blank control group was higher than that in positive control group and Sal B low, medium and high dosage group(P < 0.01), and the platelet aggregation rate was higher than that in positive control group and Sal B medium and high dosage group(P < 0.01).There was no significant difference in prothrombin time(PT) between the five groups(P>0.05), but there was significant difference in thrombin time(TT), activation of partial thromboplastin time(APTT) and fibrinogen(FIB)(P < 0.05 to P < 0.01).

ConclusionsSal B can effectively protect the cerebral ischemia rats by inhibiting platelet aggregation, improving blood hypercoagulable state, reducing the risk of thrombosis.