Determination of ligustrazine in human plasma using nanofiber-packed solid-phase extraction-high performance liquid chromatography

ObjectiveTo establish a nanofiber-packed solid-phase extraction-high performance liquid chromatography to determine the serum level of ligustrazine.

MethodsThe sample was diluted by 1% ammonia solution for 3 times, purified and enriched with activated PCAX composite anion exchange nanofiber solid-phase extraction column, eluted with deionized water, eluted with 50 μL methanol, and 20 μL sample was detected.The methanol-1% acetic acid solution(45:55, V/V) was used as mobile phase for separation on Shimazu ODS-2 column(250 mm×4.6 mm, 5 μm) with flow rate for 1.0 mL/min and detection wavelength for 295 nm.

ResultsThe linear range of this method was from 0.01 to 2.00 μg/mL, the detection limit was 0.003 μg/mL, the recovery rate was from 94.88% to 103.11%, and the relative standard deviations was from 2.6% to 4.3%.

ConclusionsThe method has the advantages of simple operation, low sample requirement and high sensitivity, and can be used to determine the content of ligustrazine in human plasma.