Effect of epimedium on the expression levels of BMP-2 and BMP-4 in cartilage tissue of rabbit model with lumbar intervertebral disc degeneration

HAN Zhong-bing; ZHANG Chang-chun; YE Yu-chen; ZHU Kun; XU Gang

doi:10.13898/j.cnki.issn.1000-2200.2019.07.003

Journal of Bengbu Medical University > 2019 > 44(7): 850-854. > DOI: 10.13898/j.cnki.issn.1000-2200.2019.07.003

HAN Zhong-bing, ZHANG Chang-chun, YE Yu-chen, ZHU Kun, XU Gang. Effect of epimedium on the expression levels of BMP-2 and BMP-4 in cartilage tissue of rabbit model with lumbar intervertebral disc degeneration[J]. Journal of Bengbu Medical University, 2019, 44(7): 850-854. DOI: 10.13898/j.cnki.issn.1000-2200.2019.07.003

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Effect of epimedium on the expression levels of BMP-2 and BMP-4 in cartilage tissue of rabbit model with lumbar intervertebral disc degeneration

Department of Orthopedics, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233004, China

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Received Date: May 30, 2018
Revised Date: March 29, 2019

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ObjectiveTo investigate the effects of epimedium on the expression levels of bone morphogenetic protein -2(BMP-2) and -4(BMP-4) in cartilage tissue of rabbit model with lumbar intervertebral disc degeneration.
MethodsThirty New Zealand white rabbits were divided into the sham operation group, model group and epimedium group (10 rabbits each group), and the lumbar intervertebral disc degeneration rabbit models in other two groups were established except for the sham operation group.The epimedium group and model group were treated with intragastric administration of 10 mg·kg^-1·d^-1 epimedium solution and same volume of 0.9% sodium chloride injection, respectively.The lumbar cartilage endplate specimens were harvested from each group after 90 d of treatment, and the morphological change of tissue was observed.The expression levels of apoptosis factor fas receptor, and BMP-2 and BMP-4 protein in cartilage endplate were determined using immunohistochemistry and Western blotting, respectively.
ResultsThe positive rates of fas receptor in model group and epimedium group were higher than that in sham operation group (P < 0.01), and the positive rate of fas receptor in epimedium group was lower than that in model group (P < 0.01).The expression levels of BMP-2 and BMP-4 protein in model group and epimedium group were lower than those in sham operation group (P < 0.01), the expression level of BMP-2 protein in epimedium group was higher than that in model group (P < 0.01), and there was no statistical significance in the expression level of BMP-4 among three groups (P>0.05).
ConclusionsEpimedium can up-regulate the expression level of BMP-2 in cartilage tissue of rabbit model with lumbar intervertebral disc degeneration, promote the proliferation and differentiation of osteoblast, but there is no experimental basis for regulating BMP-4.
- lumbar intervertebral disc degeneration,
- epimedium,
- bone morphogenetic protein

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[1]	林曦, 叶君健.骨形态发生蛋白2对退变椎间盘组织学和Ⅰ, Ⅱ型胶原的影响[J].中国组织工程研究, 2013, 17(43):7501. doi: 10.3969/j.issn.2095-4344.2013.43.001
[2]	雷涛, 权正学, 张圆, 等.联合应用BMP-2及细胞分化抑制因子1基因延缓兔腰椎间盘退变的动物实验研究[J].中国修复重建外科杂志, 2017, 31(1):73. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgxfcjwkzz201701014
[3]	张景, 张水花, 沈思, 等.T1ρ成像技术监测淫羊藿治疗早期兔膝软骨退变[J].实用放射学杂志, 2016, 32(10):1609. doi: 10.3969/j.issn.1002-1671.2016.10.033
[4]	DÄ-AZ PU, HEIN GJ, BELOTTI EM, et al.BMP2, 4 and 6 and BMPR1B are altered from early stages of bovine cystic ovarian disease development[J].Reproduction, 2016, 152(4):333. doi: 10.1530/REP-15-0315
[5]	周栩, 权正学, 罗小辑, 等.Id1基因在BMP-2维持椎间盘细胞软骨特性中的作用[J].第三军医大学学报, 2015, 37(20):2011. http://d.old.wanfangdata.com.cn/Periodical/dsjydxxb201520002
[6]	孟祥玉, 巴穆登, 艾尔肯·阿木冬.两种方法建立兔腰椎间盘退变模型[J].中国组织工程研究, 2017, 21(4):564. http://d.old.wanfangdata.com.cn/Periodical/xdkf201704012
[7]	魏文元, 武永利, 李春, 等.四逆汤饼灸对兔膝关节退变模型血清中IL-1、IL-6和TNF-α水平的影响[J].辽宁中医杂志, 2017, 44(3):615. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=lnzyzz201703055
[8]	XUE L, JIANG Y, HAN T, et al.Comparative proteomic and metabolomic analysis reveal the antiosteoporotic molecular mechanism of icariin from Epimedium brevicornu maxim[J].J Ethnopharmacol, 2016, 4(192):370. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=ff26807c990a6cbd15cfbc367c47aa92
[9]	尹宏, 钱卫庆.淫羊藿对骨质疏松型骨折局部骨形成蛋白mRNA表达的影响[J].中国骨质疏松杂志, 2012, 18(8):717. doi: 10.3969/j.issn.1006-7108.2012.08.009
[10]	杨丽, 朱晓峰, 王攀攀, 等.淫羊藿水提取物对大鼠骨髓间充质干细胞骨向分化的影响及其机制[J].中草药, 2013, 44(22):3182. http://d.old.wanfangdata.com.cn/Periodical/zcy201322016
[11]	CHURCH RH, KRISHNAKUMAR A, URBANEK A, et al.Gremlin1 preferentially binds to bone morphogenetic protein-2(BMP-2) and BMP-4 over BMP-7[J].Biochem J, 2015, 466(1):55. doi: 10.1042/BJ20140771
[12]	周慧芳, 史念珂, 雪原, 等.淫羊藿甙促进成骨细胞增殖与分化的作用及其机制[J].中华骨科杂志, 2013, 33(6):664. doi: 10.3760/cma.j.issn.0253-2352.2013.06.011
[13]	李晶, 宋敏, 罗晓, 等.淫羊藿总黄酮对去势大鼠骨组织BMP-2和OPGmRNA表达的影响[J].中华中医药杂志, 2015, 30(7):2529. http://www.cnki.com.cn/Article/CJFDTotal-BXYY201507087.htm
[14]	XU YQ, LI ZZ, YUAN L, et al.Variation of epimedins A-C and icariin in ten representative populations of Epimedium brevicornu Maxim and implications for utilization[J].Chem Biodivers, 2013, 10(4):711. doi: 10.1002/cbdv.201100424
[15]	徐无忌, 杨康, 刘思华.六味地黄丸对椎间盘体外退变模型椎间盘细胞外基质组分的影响[J].中国组织工程研究, 2017, 21(16):2495. doi: 10.3969/j.issn.2095-4344.2017.16.007
[16]	张雁儒, 张辉, 卡卡, 等.骨形态发生蛋白质-4转染骨髓间充质干细胞复合双层聚乳酸羟基乙酸共聚物支架修复兔膝关节软骨缺损[J].解剖学杂志, 2016, 39(6):667. doi: 10.3969/j.issn.1001-1633.2016.06.006
[17]	CAI M, LI G, TAO K, et al.Maohuoside A acts in a BMP-dependent manner during osteogenesis[J].Phytother Res, 2013, 27(8):1179. doi: 10.1002/ptr.4840
[18]	INDRAN IR, LIANG RL, MIN TE, et al.Preclinical studies and clinical evaluation of compounds from the genus Epimedium for osteoporosis and bone health[J].Pharmacol Ther, 2016, 162:188. doi: 10.1016/j.pharmthera.2016.01.015

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Effect of epimedium on the expression levels of BMP-2 and BMP-4 in cartilage tissue of rabbit model with lumbar intervertebral disc degeneration

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