Effect of the expression of siRNA targeting MMP-2 on the biological characteristics of colorectal cancer cells

ObjectiveTo investigate the effects of the expression of siRNA targeting MMP-2 on the biological characteristics of colorectal cancer cells.

MethodsThe expression levels of MMP-2 in colorectal cancer tissues and normal adjacent tissue was detected using Western bloting.The SW620 colorectal cancer cell was set as the control, and the MMP-2 siRNA and siRNA control were transfected into colorectal cancer cells.After 48h of transfection, the expression levels of MMP-2 in MMP-2 siRNA group, siRNA control group and control group were detected using Western bloting, and the survival rate and apoptosis rate of colorectal cancer cells in three groups were detected using MTT assay and flow cytometry, respectively.The expression levels of Bcl-2, Bax, β-catenin, C-myc and cyclin D1were detected using Western bloting.After colorectal cancer cells and Wnt/β-catenin signaling pathway were treated with the inhibitor FH535, the proliferation and apoptosis of cells in inhibitor group and untreated group were detected, and the expression levels of Bcl-2, Bax, β-catenin, C-myc and cyclin D1 were detected.

ResultsThe expression level of MMP-2 in colorectal carcinoma tissue was significantly higher than that in adjacent tissues(P < 0.01).The expression level of MMP-2 in MMP-2 siRNA group was lower than that in control group and siRNA group(P < 0.05), the survival rate of colorectal cancer cells transfected with MMP-2 siRNA was lower than that in control group(P < 0.05), and the apoptosis rate in MMP-2 siRNA group was higher than that in control group and siRNA group(P < 0.05).The levels of Bcl-2, β-catenin, C-myc and cyclin D1 in MMP-2 siRNA group were significantly lower than those in control group and siRNA control group(P < 0.05), the level of Bax in MMP-2 siRNA group was higher than that in control group and siRNA group(P < 0.01).After the Wnt/β-catenin signaling pathway was treated with the inhibitor, the survival and apoptosis rates of cells in inhibitor group were lower and higher than that in untreated group, respectively(P < 0.05), the levels of Bcl-2, β-catenin, C-myc and cyclin D1 in inhibitor group were significantly lower than that in untreated group(P < 0.01), the level of Bax in inhibitor group was significantly higher than that in untreated group(P < 0.01), which was consistent with that in colorectal cancer cells transfected with MMP-2 siRNA.

ConclusionsThe MMP-2 expression is up-regulated in colorectal cancer tissue.The proliferation of colorectal cancer cells with inhibiting MMP-2 expression is inhibited, the apoptosis of colorectal cancer cells increase, and the mechanism of which is related to Wnt/β-catenin signaling pathway.