Objective To investigate the correlation between connexin(CX) 26, CX32, CX43 and epithelial mesenchymal transformation associated protein(E-cadherin and N-cadherin), and colorectal primary carcinoma and liver metastasis.
Methods Forty colorectal cancer patients with liver metastasis diagnosed by pathological examination were investigated, and treated without chemotherapy, radiation, intervention and immunity therapy before operation.Forty colorectal cancer tissue specimens, 40 normal tissues adjacent to cancer specimens and 40 liver metastasis tissue specimens were divided into the group A, group B and group C, respectively.The expression levels of E-cadherin, N-cadherin, CX26, CX32 and CX43 in three groups were detected by immunohistochemical SP method.The relationship between the expression levels of E-cadherin, N-cadherin, CX26, CX32 and CX43 and clinicopathological features(including gender, age, depth of tumor invasion, degree of tissue differentiation and lymph node metastasis) of colorectal cancer were analyzed.The correlations of liver metastasis with levels of epithelial mesenchymal transformation, intercellular protein, CX26, CX32 and CX43 in colorectal cancer were analyzed.
Results The differences of the positive expression rate of CX43 among the three groups were statistically significant(P < 0.05), and the differences of the positive expression rate of CX26, CX32, E-cadherin and N-cadherin among three groups were not statistically significant(P>0.05).The differences of the expression levels of E-cadherin, E-cadherin, CX26, CX32 and CX43 in different sex, age, depth of tumor invasion, degree of tissue differentiation and lymph node metastasis were not statistically significant(P>0.05), and the expression level of N-cadherin was positively correlated with degree of tissue differentiation(P < 0.05).
Conclusions The expression levels of CX43 and epithelial mesenchymal transformation-associated proteins are significantly associated with colorectal primary carcinoma and liver metastasis.
DownLoad: