Effect of paeonol on the radiosensitization of esophageal cancer celland its mechanism

ObjectiveTo study the effects of paeonol on the radiosensitivity of esophageal squamous carcinoma cell lines KYSE450 and TE10, explore its molecular mechanism, and provide a new basis for the comprehensive treatment of esophageal squamous cell carcinoma with paeonol as radiotherapy sensitizing drug.

MethodsEsophageal cancer cell lines were stably cultured.After the cells were treated with different concentrations of paeonol, the CCK8 method was used to detect the proliferation of cells, the ability of colony formation was detected using colony formation assay, the apoptosis was detected using flow cytometry, and the expression of apoptosis-related protein was detected using Western blotting.

ResultsPaeonol could inhibit the proliferation of esophageal cancer cells at a concentration-dependent manner, inhibit the colony-forming ability of tumor cells after radiotherapy, and increase the apoptosis rate of tumor cells.The results of Western blotting analysis showed that the radiotherapy combined with paeonol could reduce the expression of Bcl-2, and increase the expression levels of Bax, caspase-3, cleaved caspase-3 and PARP.

ConclusionsPaeonol combined with radiation therapy can inhibit the expression of Bcl-2, up-regulate the expression levels of Bax, caspase-3, cleaved caspase-3 and PARP, and the growth of esophageal cancer cells in vitro is inhibited to improve the radiosensitization.