Effect of Biling Qutong prescription on the levels of HMGB1 and FOXO3 in kidney of diabetic gout model rats

ObjectiveTo observe the effects of Biling Qutong prescription(Biling prescription) on the levels of high mobility group B1(HMGB1) and FOXO3 in kidney of diabetic gout model rats.

MethodsForty healthy rats were divided into the normal control group, model group, Biling group, indomethacin group and pioglitazone group(8 rats in each group).Except the normal control group, the other groups were fed with high-fat diet combined with low-dose streptozotocin 35 mg/kg once a day, and the blood glucose ≥"16.7 mmol/L was set as the standard of the diabetes model.After 4 days, the 0.9% sodium chloride solution was injected into the joint cavity of rats to establish the gout model.After the model was successfully established, the Biling group, indomethacin and pioglitazone group were continuously treated with relative drug for 21 days.The normal control group and model group were given the same amount of 0.9% sodium chloride solution.After 21 days, the levels of blood glucose, uric acid and blood uric acid, and expression levels of HMGB1 and FOXO3 in kidney were detected.

ResultsCompared with the normal control group, the levels of blood glucose, serum uric acid, triacylglycerol(TG), total cholesterol (TC), low-density lipoprotein cholesterol(LDL-C) significantly increased(P < 0.01), and the expression levels of HMGB1 and FOXO3 in kidney significantly increased in model group(P < 0.01).Compared with the model group, the levels of blood glucose and serum uric acid decreased, and the expression levels of HMGB1 and FOXO3 in kidney significantly decreased in Biling group, indomethacin and pioglitazone group(P < 0.05 to P < 0.01).Compared with the model group, the levels of TG, TC and LDL-C in Biling group and pioglitazone group decreased(P < 0.05 to P < 0.01), and the levels of TC and LDL-C in indomethacin group decreased(P < 0.05 and P < 0.01).

ConclusionsBiling prescription has good effects in lowering blood glucose, regulating lipid and lowering blood uric acid, the action mechanism of which may be related to the intervention of the high expression of HMGB1 and FOXO3 in kidney of diabetic gout rats, and which is of great significance for the prevention and treatment of kidney disease induced by diabeticgout.