Effect of sodium acetate Ringer's solution on inflammatory mediators and NF-κB, MAPK signaling pathway in myocardial tissue of hemorrhagic shock rats

ObjectiveTo explore the effect of sodium acetate Ringer's solution on inflammatory mediators and NF-κB, MAPK signaling pathway in myocardial tissue of traumatic hemorrhagic shock rats.

MethodsThirty two healthy SD rats were randomly divided into shock without resuscitation group (CR group), resuscitation with 0.9% sodium chloride solution group (NR group), resuscitation with sodium lactate Ringer's solution group (LR group), and resuscitation with sodium acetate Ringer's solution group (AR group) (eight rats in each group).The shock model was made in each group (the average arterial pressure maintained at 30 mmHg).CR group was not resuscitated, NR, LR and AR group were resuscitated with corresponding solution at 60 min after shock, and observed for 4 h after resuscitation.The myocardial tissue of rats in each group was taken for morphological observation, and the expression level of related cytokines and proteins was detected.

ResultsThe expression level of TNF-α mRNA of myocardial tissue in AR, LR and NR group was significantly lower than that in CR group (P < 0.01), the expression level of IL-10 mRNA in AR and LR group was significantly higher than that in CR group (P < 0.01 and P < 0.05), and the expression level of IL-4 mRNA in AR group was significantly higher than that in LR, NR and CR group (P < 0.01).The relative expression of p65 (Ser536) phosphorylation, p65(Lys310) acetylation and JNK phosphorylation protein in AR, LR and NR group was lower than that in CR group (P < 0.05 to P < 0.01), and which in AR group was lower than that in NR and LR group (P < 0.05 to P < 0.01).The relative expression of MKP-1 acetylation protein in AR, LR and NR group was higher than that in CR group (P < 0.05 to P < 0.01), and which in AR and LR group was significantly higher than that in CR group (P < 0.01).

ConclusionsSudium acetate Ringer's solution for resuscitating hemorrhagic shock rats may protect myocardial tissue by regulating the expression of NF-κB and MAPK signaling pathway, and reduce the inflammatory response of myocardial tissue to a certain extent.