Effect of PD-L1 on the proliferation,invasion and migration of hepatocellular carcinoma cells

ObjectiveTo investigate the effects of PD-L1 in the proiferation, invasion and migration of hepatacellular carcinorma cells.

MethodsThe effective PD-L1 gene siRNA interference fragment was transfected into the human hepatocellular carcinoma HepG2 cells to down-regulate PD-L1 expression.The expression levels of PD-L1 gene and protein in HepG2 cells were detected using the real-time PCR and Western blotting, resepctively.The cells proliferation and fusion rate of HepG2 cells were observed using the MTT assay and cell scratch test, respectively.The invasion experiment was implemented using Transwell chamber, and the migration and invasion ability of HepG2 cells were observed.

ResultsAfter the expression level of PD-L1 gene was down-regulated, the expression levels of PD-L1 gene and protein in HepG2 cells, and proliferation, migration and invasion abilities of HepG2 cells in PD-L1 interference group were significantly lower than those in negative control group and blank control group(P＜0.01), and there was no statistical significance in the above indicators between the negative control group and normal control group(P＞0.05).

ConclusionsAfter the down-regulation of PD-L1, the proliferation, migration and invasion abilities of the human hepatocellular carcinoma cells decrease to a certain extent, and PD-L1 may become a new gene target in the immunotherapy of hepatocellular carcinoma.