Effect of lentivirus-mediated silencing SOCS3 gene on the expression of related insulin pathway proteins in diabetic rats

ObjectiveTo construct the diabetic rat model, and investigate the effects of lentivirus-mediated silencing the suppressors of cytokine signaling 3(SOCS3) gene on the expression of related insulin pathway proteins in diabetic rats.

MethodsForty SD rats model of type 2 diabetes were constructed, and randomly divided into the blank control group, blank vector group and observation group(10 rats each group).The blank control group was not treated, the empty lentiviral vector was injected into the tail of rats of blank vector group, and the SOCS3 RNAi lentiviral vector was injected into the tail of rats of observation group.The levels of blood glucose, blood lipid and insulin in three groups were measured after 4 weeks of injection.The expression levels of SOCS3, IRS1, IRS2, STAT5b, JAK2 and STAT3 mRNA were detected using RT-PCR, and the expression levels of SOCS3, IRS1, pIRS1, IRS2, pIRS2, STAT5b, pSTAT5b, JAK2, pJAK2, STAT3 and pSTAT3 protein were detected using Western blotting.

ResultsAfter 4 weeks of injection, the levels of fasting blood glucose, insulin, TG, LDL-C and TC in observation group were significantly lower than those in blank control group and blank vector group(P＜0.01), and the level of HDL-C in observation group was significantly higher than that in blank control group and blank vector group(P＜0.01).The expression levels of SOCS3 and STAT5b gene mRNA in observation group were lower than those in blank control group and blank vector group(P＜0.01), the expression levels of IRS1, IRS2 and JAK2 mRNA in observation group were higher than those in blank control group and blank vector group(P＜0.05 to P＜0.01), and the differences of the expression level of STAT3 mRNA among three groups were not statistically significant(P>0.05).The expression levels of SOCS3, STAT5b and pSTAT5b protein in observation group were significantly lower than those in blank control group and blank vector group(P＜0.01), the expression levels of IRS1, pIRS1, IRS2, pIRS2, JAK2, pJAK2 and pSTAT3 protein in observation group were significantly higher than those in blank control group and blank vector group(P＜0.01), and the differences of the levels of STAT3 protein among three groups were not statistically significant(P＞0.05).

ConclusionsSilencing the SOCS3 gene can reduce the blood glucose level in diabetic rats, and the SOCS3 gene may mediate insulin resistance through related pathways.