Formulation screening and preparation process optimization of 3-bromopyruvate liquid crystalline nanoparticles

ObjectiveTo screen the formulation and preparation process optimization of 3-bromopyruvate liquid crystalline nanoparticles(3-BP-LCNP).

Methods3-BP-LCNP were prepared by injection method combined with high pressure homogenization.The particle size, encapsulation rate and drug loading were set as the evaluation index, the orthogonal design and single factor analysis were used to optimize the prescription screening and preparation process.The particle size and potential were measured using Malvern particle size meter and the encapsulation rate and drug loading were investigated by dialysis method.

ResultsThe optimal formulation and preparation process of 3-BP-LCNP was monooleate:poloxamer 407 at 8:1(the total mass kept at 1 g), the dispersed phase was 25 mL, the 3-BP input was 26.67 mg, and the cycle was 9 times at 14 900 psi.The results showed that the average particle diameter was 192.7 nm, the average encapsulation efficiency was 72.53%, and the drug loading was 2.71%.

ConclusionThe preparation method and process of 3-BP-LCNP are stable and feasible by using the injection method combined with high pressure homogenization.