Study on the chemical fingerprint features and quantitative analysis of 4 components of Trichosanthis Fructus dropping pills

ObjectiveTo study on the chemical fingerprint characteristics of Trichosanthis Fructus dropping pills, and quantitative analysis of 4 components.

MethodsThe chemical fingerprints of 10 batches of Trichosanthis Fructus dropping pills were established by HPLC-PDAD, and the similarity of Trichosanthis Fructus dropping pills was calculated using the similarity evaluation system of chromatographic fingerprint of TCM.The contents of uracil, hypoxanthine, guanosine and adenosine in Trichosanthis Fructus dropping pills were quantitatively analyzed using the standard curve method.

ResultsThe similarity of 10 batches of Trichosanthis Fructus dropping pills were 0.99(Mark peak matching) and 0.97(full spectrum peak matching).The contents of uracil, hypoxanthine, guanosine and adenosine in Trichosanthis Fructus dropping pills were 0.06%, 0.03%, 0.11% and 0.17%, respectively.The maximum RSD values of precision, stability, repeatability and sample recovery rate were 2.24%, 3.55%, 4.23% and 4.44%, respectively, which could meet the requirements of analysis.

ConclusionThe established analysis methods of the chemical fingerprint features and quantitative analysis of 4 components of Trichosanthis Fructus dropping pills can be used in the quality control of Trichosanthis Fructus dropping pills.