Role and clinical significance of Fhit gene in the proliferation and invasion of endometrial carcinoma

ObjectiveTo explore the expression level of Fhit gene in endometrial carcinoma, and observe its effects on the proliferation, invasion and migration of endometrial carcinoma cells through the RNA activation up-regulating the expresson of Fhit.

MethodsThe expression levels of Fhit protein in 135 endometrial carcinoma tissues and 40 normal endometrial tissues were detected using immunohistochemistry and Western blotting.The correlation between Fhit expression and clinicopathological features was analyzed.The Fhit-saRNA expression vector was constructed, and transfected into endometrial carcinoma ISK cell line, and the endometrial carcinoma cell line with Fhit up-regulating expression was established.The transfection effects were verified using Western blotting and RT-PCR.The differences of the proliferation, migration and invasion abilities between before and after transfection were analyzed using CCK-8 and Transwell assays.

ResultsThe results of immunohistochemistry and Western blotting showed that the expression levels of Fhit in endometrial carcinoma tissue significantly reduced or even missed compared with normal endometrial tissue(P < 0.01).The results of CCK-8 showed that the growth of endometrial carcinoma significantly slowed down after the up-regulating of Fhit expression, and the difference of which between transfection group and non-transfection group was statistically significant(P < 0.01).The results of Transwell assay showed that the migration and invasion abilities of endometrial carcinoma cells after the level of Fhit up-regulating significantly weakened, and the number of transmembrane cells in blank control group were significantly higher than that in transfection group(P < 0.01).

ConclusionsCompared with normal endometrial tissues, the expression level of Fhit in endometrial carcinoma tissues is lower, and related to the clinicopathological features.After up-regulating the Fhit expression by RNA activation in endometrial carcinoma cell line, the abilities of proliferation, migration and invasion of cells are significantly inhibited.