Effect comparison of two methods in the detection of serum amyloid A protein

ObjectiveTo compare the effects between immunovelocity nephelometry and immunofluorescence chromatography in the detection of serum amyloid protein A(SAA).

MethodsThe serum levels of SAA of inhospital patients(observation group) and healthy physical examinees(control group) were detected using the full-automatic specific protein detector(immunovelocity nephelometry) and fluorescence immunoquantitative analyzer (immunofluorescence chromatography), and the detection results were compared between two methods.

ResultsThe serum levels of SAA deetcted by two methods in observation grop were higher than those in control group(P < 0.01).The serum level of SAA in observation group detected by immunovelocity nephelometry was higher than that of immunofluorescence chromatography(P < 0.01), while the difference of the serum level of SAA in control group was not statistically significant between two detection methods(P>0.05).The sensitivities and specificities of immunovelocity nephelometry and immunofluorescence chromatography were 53.00% & 57.00% and 92.00% & 93.00%, respectively, and the differences of those were not statistically significant between two methods(P>0.05).The lower limit probability of SAA detected by fluorescence immunochromatography in control group(83.00%, 83/100) was higher that of immunovelocity nephelometry(42.00%, 42/100) (χ²=35.86, P < 0.01).The fluorescence immunochromatography in the detection of low value was more stable.

ConclusionsThe immunovelocity nephelometry and immunofluorescence chromatography can be used for clinical detection, the latter has better stability.