Analysis of drug resistance rate and homology of carbapenem-resistant Klebsiella pneumoniae

ObjectiveTo analyze the drug-resistant genes and epidemiological characteristics of carbapenem-resistant Klebsiella pneumoniae.

MethodsFourteen strains of carbapenem-resistant Klebsiella pneumoniae were collected from January to August 2018, and the strain identification and drug susceptibility test were implemented using the VITEK Compact system.The modified Hodge test was used to detect the carbapenems, the PCR test was used to detect the drug-resistant genes, and the homology of the strains was analyzed using intergene repeated sequence polymerase chain reaction(ERIC-PCR).

ResultsFourteen strains of carbapenem-resistant Klebsiella pneumoniae were highly resistant to penicillins, carbapenems, aztreonam and cephalosporins.Eleven strains of Klebsiella pneumoniae were positive in the modified Hodge test.The positive KPC-2 gene in 12 strains of Klebsiella pneumoniae were identified by PCR.The 14 strains could be classified into 3 genotypes, the type A was main, which was mainly isolated from ICU and neurosurgery.

ConclusionsThe carbapenem-resistant Klebsiella pneumoniae shows the severe multi-drug resistance, mainly producing KPC enzyme and type A.