Role of long non-coding RNA LINC00173 in paclitaxel-resistant breast cancer cells

ObjectiveTo investigate the effects of long non-coding RNA LINC00173 on the proliferation, invasion and migration of paclitaxel-resistant breast cancer cells, and its possible mechanism of action.

MethodsThe expression levels of LINC00173 in breast cancer cell lines(MCF-7, MDA-MB-231 and SKBR-3) and normal breast cancer epithelial cell lines(MCF-10A) were detected using qRT-PCR.The LINC00173 overexpression vector and interference fragment were constructed, and transfected into breast cancer cell lines, respectively.The qRT-PCR was used to detect the expression level of transfected LINC00173.The proliferation ability of breast cancer cells was detected using CCK8 assay.The transwell invasion and scratch assay were used to detect the invasion and migration of cells.The expression levels of CyclinD1, β-catenin and P-gp proteins were detected using Western blotting.

ResultsCompared with MCF-10A cells, the expression level of LINC00173 in breast cancer cell line was low.After MCF-7 cells transfected with interference fragments and MCF-7/PR resistant cell lines transfected with overexpression vector of si-LINC00173, the expression level of si-LINC00173 decreased compared with the negative control group(si-NC).Compared with the LV-NC group, the overexpression of LV-LINC00173 increased, and the difference of which was statistically significant(P < 0.01).After interfering the expression of LINC00173, the proliferation and migration of MCF-7 cells enhanced(P < 0.01).After overexpression of LINC00173, the proliferation, invasion and migration of MCF-7/PR cells decreased(P < 0.01).After the interfering with LINC00173, the expression levels of CyclinD1, β-catenin and P-gp in MCF-7 cells significantly increased(P < 0.01).After the overexpression of LINC00173, the expression levels of CyclinD1, β-catenin and P-gp in MCF-7/PR decreased significantly(P < 0.01).

ConclusionsThe decreasing expression level of LINC00173 in breast cancer cells may participate in the process of paclitaxel-resistance by regulating β-catenin expression and its occurrence, development and invasion.LINC00173 is a new molecule involved in the development of breast cancer, and which can provide a new theoretical basis for clinical diagnosis and treatment of breast cancer.