Analysis of the influencing factors of mobilization and collection of autologous hematopathy in patient with malignant blood disease

ObjectiveTo explore the effects of various mobilization schemes, age, chemotherapy cycle, disease state and other factors on the autologous peripheral blood stem cell mobilization and collection.

MethodsThe clinical data of 31 patients with acute leukemia(AL), Non-Hodgkin's lymphoma(NHL) and multiple myeloma(MM) were retrospectively analyzed.The patients with Granulocyte Colony-Stimulating Factor(G-CSF) mobilization scheme, large dose VP-16(Etoposide) combined with G-CSF mobilization scheme and Cyclophosphamide(CTX) combined with G-CSF mobilization scheme were divdied into the the G-CSF group, VP-16 group and CTX group, respectively.The number of cells, changes of hematological indexes and adverse reactions among three groups were compared.The effects of age, gender, disease status, chemotherapy cycle, treatment to mobilization time on stem cell collection were analyzed.

ResultsA total of 59 times were collected from 31 people, and the (5.7±1.6)×10⁸/kg mononuclear cells and (2.1±0.7)×10⁶/kg CD34⁺ cells were obtained.The number of median day from mobilizating to collecting in G-CSF group was 4 days, but the CD34⁺ cell production in G-CSF group was lower than that in other two groups(P < 0.05).There was no statistical significance in the number of CD34⁺ cells between the VP-16 group and CTX group(P>0.05), and the number of median day from mobilizating to collecting in VP-16 group and CTX group were 12 days.The platelet level in CTX group was lower than that in VP-16 group(P < 0.05).Five cases with ranulocytopenia complicaed with fever(>38 ℃) in VP-17 group were found after chemotherapy, while 3 patients in the CTX group were identified.The number of CD34⁺ cells collection in male patients was higher than that in female patients, which in patients with ≤55 years old was higher than that in patients with >55 years old, which in patients with ≤8 chemotherapy courses was higher than that in patients with >8 chemotherapy courses, and which in CR patients was better than that in PR patients(P < 0.05).

ConclusionsChemotherapy combined with G-CSF can mobilize more CD34⁺ cells, and the platelet level in the high-dose VP-16 + G-CSF Mobilization scheme is higher, and the safety of collection is more guaranteed.The success rates in male, age ≤55 years old and course of chemotherapy ≤8 courses patients are higher.