The relationship between the interleukin-1 gene polymorphism and Helicobacter pylori infection, and atherosclerosis in healthy middle-aged population

ObjectiveTo investigate the effects of the gene polymorphism of IL-1-511 or IL-1RN VNTR on atherosclerosis in Helicobacter pylori (HP) infection aggravation healthy middle-aged people.

MethodsThe genotypes loci polymorphism of IL-1 511 or IL-1RN VNTR in 227 healthy middle-aged people were determined using polymerase chain reaction(PCR)-restriction digestion analysis.The HP IgG, and CagA and VacA antibody were detected using ELISA, and the degree of atherosclerosis was assessed using the ankle-brachial index(ABI).

ResultsThe main polymorphism of IL-1β-511 were CT(64.8%) and TT(22.3%), respectively.The allele frequency of four-repeat(IL-1RN*1) was 68.7%, and two repeat(IL-1RN*2) alleles was 29.2% in IL-1RN VNTR.The difference of ABI in subjects with different Il-1 gene polymorphisms was not statistically significant(P>0.05).The ABI in positive HP subjects was significantly lower than that in negative HP subjects(P < 0.01).After adjusting the age, sex, hypertension, diabetes, smoking status, BMI, triglyceride and interleukin-1 Gene polymorphism, the difference of the ABI between positive and negative HP subjects was not statistically significant(P>0.05), and the positive HP CagA antibody was a low risk factor of ABI(P < 0.01).

ConclusionsThe HP infection, especially CagA antibody-positive HP infection, may be a risk factor of atherosclerosis in the middle-aged Han population in southern China, and there is no significant correlation between it and polymorphisms of two IL-1 gene sites.