Effect of lncRNA TUG1 regulating miRNA-145 expression on the proliferation, migration and invasion of colon cancer cells

ObjectiveTo explore the effects of lincRNA TUG1 regulating miRNA-145 on the proliferation, migration and invasion of colon cancer cells.

MethodsHuman colon cancer cell line HCT116 was divided into 6 groups, and cultured for 48 h.The expression levels of TUG1 and miRNA-145 in each group were detected using qRT-PCR.The proliferation of cells was detected using MTT assay, and the ability of cell migration and invasion were detected using scratch test and Transwell invasion test.The targeting relationship between TUG1 and miRNA-145 was verified by dual-luciferase reporter gene assay.

ResultsCompared with human normal colonic mucosal epithelial cell line NCM460, the relative expression level of TUG1 in colon cancer cell line HCT116 significantly increased, while the relative expression level of miRNA-145 significantly decreased(P < 0.01).There were binding sites for miRNA-145 and TUG1 3'-UTR.Compared with the si-con group, the TUG1 expression level and proliferation activity of HCT116 cells in the TUG1 siRNA group significantly decreased, the relative width of cell scratch significantly increased, and the number of invaded cells significantly decreased(P < 0.05).Compared with the miRNA NC group, the miRNA-145 expression level in the miRNA-145 mimic group significantly increased, the proliferation activity of HCT116 cells significantly decreased, the relative width of cell scratch significantly increased, and the number of invaded cells significantly decreased(P < 0.05).Compared with the SI-CON group, the expression level of miRNA-145 in TUG1 siRNA group significantly increased(P < 0.05).Compared with the si-TUG1+miRNA NC group, the expression level of miRNA-145 in the TUG1 siRNA+ mirNA-145 inhibitor group significantly reduced(P < 0.05), the proliferation activity of HCT116 cells significantly enhanced, the relative width of cell scratche significantly reduced, and the number of invaded cell significantly increased(P < 0.05).

ConclusionsThe expression level of TUG1 is up-regulated and the expression level of miRNA-145 is down-regulated in colon cancer HCT116 cells.Interfering with the expression of TUG1 can inhibit the proliferation, migration and invasion of colon cancer HCT116 cells by targeting up-regulation of miRNA-145 expression.