Effect of interleukin-34 on the proliferation of rat stem cells from the apical papilla

ObjectiveTo investigate the effects of interleukin-34(IL-34)on the proliferation of rat stem cells from the apical papilla(SCAP).

MethodsThe rat SCAP were isolated and cultured using enzyme digestion method.The surface marking molecules of SCAP were identified using flow cytometry, and the Alizarin red staining was used to identify the osteogenic potential of SCAP.The cells were divided into the experimental group(cell culture medium with different concentrations of IL-34)and control group(cell culture medium without IL-34).The effects of IL-34 on the proliferation of SCAP were detected using MTT assay.

ResultsThe results of MTT assay showed that the proliferation of SCAP was promoted at the concentration of 25 ng/mL, 50 ng/mL and 100 ng/mL of IL-34, especially the 50 ng/mL of IL-34.The proliferation of SCAP was inhibited at the concentration of 200 ng/mL, 400 ng/mL and 600 ng/mL of IL-34.

ConclusionsIL-34 can regulate the proliferation of SCAP.The low concentration of IL-34 can promote the proliferation of SCAP, while the high concentration of IL-34 can inhibit the proliferation of SCAP.