Effect of IL-33 and its receptor ST2 on the proliferation, apoptosis, migration and invasion of human ovarian cancer ES-2 cells

ObjectiveTo investigate the effects of interleukin-33(IL-33) and its receptor sulfotransferase(ST2) on the proliferation, apoptosis, migration and invasion of human ovarian cancer ES-2 cells.

MethodsThe effects of IL-33 and si-ST2 on the survival rate of ES-2 cells were detected using MTT assay, and the cells were divided into the control group, IL-33 group, si-ST2 group and IL-33+si-ST2 group.The OD value in each group was detected.The effects of IL-33 and si-ST2 on the apoptosis of ES-2 cells were detected using flow cytometry, and the number of apoptotic cells in each group was counted.The effects of IL-33 and si-ST2 on the migration and invasion of ES-2 cells were detected using the cell migration and invasion experiments.

ResultsThe differences of cell survival rates among the IL-33 group, si-ST2 group, IL-33+si-ST2 and control group were statistically significant(P < 0.01).The cell survival rate in IL-33 group was higher than that in control group, the cell survival rate in si-ST2 group was lower than that in control group, the cell survival rate in IL-33+si-ST2 group was significantly higher than that in si-ST2 group, and the cell survival rate in IL-33 group was significantly higher than that in si-ST2 group(P < 0.01).The cell apoptosis rate in IL-33 group was lower than that in control group, the cell apoptosis rate in si-ST2 group was higher than that in control group and IL-33 group, and the cell apoptosis rate in IL-33+si-ST2 group was lower than that in si-ST2 group, and higher than that in IL-33 group(P < 0.01).The differences of the cell migration rate and invasion rate among the IL-33 group, si-ST2 group, IL-33+si-ST2 group and control group were statistically significant(P < 0.01).The migration and invasion rates of cells in IL-33 group were lower than those in control group, the migration and invasion rates of cells in si-ST2 group were higher than those in control group, and the migration and invasion rates of cells in IL-33+si-ST2 group were higher than those in IL-33 group, and lower than those in si-ST2 group(P < 0.01).

ConclusionsIL-33 can promote the proliferation, and inhibit the apoptosis, migration and invasion of ES-2 cells.Silencing ST2 can inhibit the proliferation, and promote the apoptosis, migration and invasion of ES-2 cells.IL-33 affects the proliferation, apoptosis, migration and invasion of ES-2 cells through ST2 pathway.