Study on the therapeutic dose of 5-fluorouracil in a new generation PDX model of colon cancer

ObjectiveTo establish the patient-derived tumor xenografts(PDX)model of colorectal cancer(CRC), and explore the relationship among the anticancer effects, toxicity and dose of 5-fluorouracil(5-Fu).

MethodsThe fresh human CRC tissues were subcutaneously transplanted into immunodeficient mice to establish a PDX model, and the traceability of the test model was detected.The mice were treated with 5, 10, 20, 40 and 60 mg/kg of 5-Fu, respectively, and the tumor volume, body mass and mortality were measured.

ResultsThe PDX model was successfully established, and the traceability was 99.96%.The tumor inhibition rates of 5, 10, 20, 40 and 60 mg/kg of 5-Fu were 33%, 49%, 68%, 92% and 100%, respectively.Among them, the body weight of mice significantly lost in 40 mg/kg dose group, and the mortality of 60 mg/kg dose group was 100%.

ConclusionsThe efficacy and toxicity of 5-Fu are closely related to the doses, and the 10-20 mg/kg of 5-Fu is suitable for the efficacy evaluation of PDX model.