Effect of LncRNA PCBP1-AS1 on the proliferation, invasion and apoptosis of oral squamous carcinoma cells

ObjectiveTo investigate the effects of long-chain non-coding RNA PCBP1-AS1(LncRNA PCBP1-AS1)on the proliferation, invasion and apoptosis of oral squamous carcinoma cells(OSCC)cells, and its possible mechanism.

MethodsThe expression level of PCBP1-AS1 in OSCC cells was detected using Real-time quantitative polymerase chain reaction(qRT-PCR).The pcDNA-PCBP1-AS and pcDNA-control were transfected into the luminal squamous cell carcinoma CAL-27 cells using Lipofectamine 2000.The proliferation ability, invasive ability and apoptotic rate of CAL-27 cells were detected using MTT assay, Transwell experiments and flow cytometry, respectively.

ResultsCompared with the normal oral epithelial cells, the expression levels of PCBP1-AS1 in oral squamous cell carcinoma cells CAL-27, Tca8113, and KB significantly decreased(P < 0.01).The overexpression of PCBP1-AS1 could significantly inhibit the proliferation and invasion of CAL-27 cells(P < 0.01), and promote apoptosis of cells(P < 0.01).The overexpression of PCBP1-AS1 could significantly inhibit the protein expression levels of CDK1, MMP-2, Bcl2, p-JAK2 and p-STAT3(P < 0.01), and promote the protein expression of Bax(P < 0.01).

ConclusionsThe overexpression of PCBP1-AS1 can inhibit the proliferation and invasion of OSCC cells, and induce its apoptosis, and the mechanism of which may be related to the inhibition of JAK2/STAT3 signaling pathway activation.