Expression level and clinical significance of GOLPH3 and VEGF in cervical cancer

ObjectiveTo detect the expression levels of Golgi phosphoprotein 3(GOLPH3)and vascular endothelial growth factor(VEGF)in cervical cancer tissues, and explore their relationship in invasion and metastasis of cervical cancer.

MethodsThe expression levels of GOLPH3 and VEGF proteins in cervical carcinoma tissues and para-cervical cancer tissues of 43 patients were detected using immunohistochemical SP method.The correlations of GOLPH3 and VEGF levels in cervical cancer tissues were analyzed using Pearson correlation analysis.

ResultsThe positive expression rates of GOLPH3 and VEGF in cervicer cancer tissues were 72.1% and 74.42%, respectively, which were significantly higher than those in para-cervical cancer tissues(37.21% and 32.56%)(P < 0.01).The expression levels of GOLPH3 and VEGF in cervical cancer tissue were significantly correlated with the tumor size, differentiation, surgical and pathological stage, and lymph node metastasis(P < 0.05 to P < 0.01), and not significantly correlated with the age and pathological type of patients(P>0.05).The results of immunohistochemistry showed that the positive expression rate of VEGF in cervical cancer tissues with positive GOLPH3 expression group(83.87%)was significantly higher than that in negative GOLPH3 expression group(33.33%)(P < 0.01).The expression rate of GOLPH3 was positively correlated with VEGF expression in cervical cancer tissues(r=0.494, P < 0.01).

ConclusionsThe overexpression of GOLPH3 promotes the angiogenesis in cervical cancer tissues by upregulating the expression of VEGF, which leads to the invasion and metastasis of cervical cancer.