Investigation of the human papilloma virus infection and genotype distribution of gynecological patients in a hospital of Bengbu, Anhui province

ObjectiveTo investigate the infection and genotype distribution of human papilloma virus(HPV)in female cervical exfoliative cells in gynecological patients in a hospital of Bengbu, Anhui province.

MethodsThe cervical exfoliated cells from 4 773 gynecological patients in the Second Affiliated Hospital of Bengbu Medical College from 2016 to 2017 were collected.Twenty-three HPV subtypes were examined using PCR reverse dotblot hybridization technique.

ResultsAmong the 4 773 samples, 1 069 HPV positive cases were detected(including 943 cases of high-risk HPV, 88 cases of low-risk HPV, and 38 cases of high-low risk HPV), and the total infection rate of HPV was 22.40%.Among these positive samples, the single-subtype infection rate was 81.20%(868 cases), and the multiple-subtype infection rate was 18.80%(201 cases).HPV16(280 cases, 26.19%), HPV52(198 cases, 18.52%), HPV58(109 cases, 10.20%), HPV33(84 cases, 7.86%)and HPV56(81 cases, 7.58%)were the top five high-risk subtypes.The differences of the infection rates among different age groups were statistically significant(P < 0.01).The infection rates in age≥55 group, age < 25 group, 35>age≥25 group, 45>age≥35 group and 55>age≥45 group were 31.96%, 25.41%, 20.62%, 21.76% and 21.55%, respectively.

ConclusionsThe single high-risk infection is the main type of HPV infection in gynecological patients, HPV16, HPV52 and HPV58 are the top three prevalent subtypes, and the HPV infection rate in female with age more than 55 years is higher.