ObjectiveTo investigate the effects of all-trans retinoic acid(ATRA) on the radiosensitivity of lung adenocarcinoma cell line H1299, and explore its possible molecular mechanisms.
MethodsMTT assay was used to determine the effects of ATRA on the survival rate of H1299 cells, the radiosensitivity of H1299 cells was detected using plate cloning formation experiment, the H1299 cell cycle was detected using flow cytometry and the protein expression levels of survivin and NF-κB in the cells were detected using Western blotting.
ResultsThe H1299 cells could be inhibited by ATRA at different concentrations, and the best concentration of which was 10 μmol/L(P < 0.05).Compared with the cells treated with ATRA alone, 10 μmol/L ATRA combined with different doses of radiation, the inhibition rate of cell growth significantly increased(P < 0.01), the number of cell apoptosiss increased(P < 0.01), and the total apoptosis rate of ATRA combined with radiation was significantly higher than that of ATRA alone(P < 0.01).Compared with the control group, radiation group, and ATRA group, the proportion of G0/G1 phase in ATRA combined with radiation group significantly increased(P < 0.01).Compared with the radiation group, the cell survival fraction in ATRA combined with radiation group decreased, and ATRA could increase the radiosensitivity of lung adenocarcinoma H1299 cells with a sensitization ratio of 1.406.The results of Western blotting showed that the expression levels of survivin and NF-κB in H1299 cells significantly decreased in ATRA combined with radiation group(P < 0.01).
ConclusionsATRA can increase the radiosensitivity of lung adenocarcinoma H1299 cells, and the mechanism may be related to ATRA directly inhibiting the proliferation of H1299 cells, promoting the apoptosis of H1299 cells and down-regulating the survivin protein and NF-κB protein expression.
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