Influence of IL-10 and CYP3A5 gene polymorphisms on the dose-adjusted trough blood concentrations of tacrolimus in Chinese renal transplant recipients during the stable period

ObjectiveTo investigate the influence of interleukin 10(IL-10) and CYP3A5 genetic polymorphism on the dose-adjusted trough blood concentrations(C₀/D) of tacrolimus in Chinese renal transplant recipients during the stable period.

MethodsA total of 149 renal transplant recipients were enrolled in the analysis.Kompetitive allele specific PCR was used to detect the IL-10-819 C/T, -592 C/A, -1082 G/A and CYP3A5 6986 A/G gene polymorphisms.Tacrolimus trough level(C₀) was determined by chemiluminesent immunoassay assay.

ResultsAmong 149 renal transplant patients, IL-10-592 C/A and -1082 G/A gene polymorphisms had no significant effect on C₀/D of tacrolimus.But there was a significant correlation between -819C/T and tacrolimus C₀/D, C₀/D of tacrolimus for patients with IL-10-819 TT was higher than that of those with -819 TC/CC(P < 0.05).And C₀/D of tacrolimus in the CYP3A5 non-expressing group(6986 GG genotype) was higher than those in the CYP3A5 expressing group(6986 GA/AA genotype)(P < 0.05).Moreover, C₀/D of tacrolimus of IL-10-819 TT genotype was higher than that of -819 TC/CC genotype in the CYP3A5 expressing group, which showed the similar results in the CYP3A5 non-expressing group(P < 0.05).

ConclusionsIL-10-819 C/T and CYP3A5 6986 A/G gene polymorphisms are associated with the C₀/D of tacrolimus in renal transplant patients during the stable period, and IL-10-819 TT has the greater effect on the C₀/D of tacrolimus in CYP3A5 6986 GG patients.