Analysis of the antibody detection in 215 neonates with hyperbilirubinemia

ObjectiveTo analyze the antibody detection results of three hemolysis tests in 215 neonates with hyperbilirubinemia(NHB), and explore the clinical significance of early detection.

MethodsThe venous blood in 215 neonates diagnosed with NHB within 3 days after birth were collected, the blood group test, direct resistance test, free test and release test were performed, and the results were analyzed.

ResultsAmong 215 children with NHB, 97 cases were diagnosed with hemolytic disease of the newborn(HDN).The percentages of type A, type B and type O in the HDN group were 52.58%(51/97), 45.36%(44/97) and 2.20%(2/97), respectively, and the differences of composition ratio of type A, type B and type O between HDN group and non-HDN group were statistically significant(χ²=61.49, P < 0.01).The positive rates of the release test, free test and direct resistance test were 100%(97/97), 78.35%(76/97) and 16.50%(16/97), respectively.The differences of the detection rates among three methods were statistically significant(χ²=160.04, P < 0.01), and which in release test, free test and direct resistance test gradually decreased in turn.

ConclusionsThrough rapid detection of HDN microcolumn gel card, the timely diagnosis can be made, so as to select appropriate methods for positive symptomatic treatment.