Expression of high mobility group protein B1 in breast invasive ductal carcinoma tissues and its relationship with prognosis

ObjectiveTo analyze the expression level of high mobility group protein B1(HMGB1) in breast invasive ductal carcinoma tissues, and its relationship with prognosis.

MethodsThe clinicopathological data of 139 female patients with breast invasive ductal carcinoma were collected.Immunohistochemistry was used to detect the expression levels of HMGB1 in breast invasive ductal carcinoma tissue and adjacent normal tissue.The relationship between HMGB1 expression level and clinicopathological characteristics in breast invasive ductal carcinoma was analyzed, and the relationship between HMGB1 expression level and prognosis in patients with breast invasive ductal carcinoma was evaluated.

ResultsThe nuclear high expression rate and cytoplasmic positive rate of HMGB1 in breast invasive ductal carcinoma tissues were 80.58% and 16.55%, respectively, which were significantly higher than those in adjacent normal tissues(46.04% and 0.00%) (P < 0.01).The cytoplasmic positive expression rates of HMGB1 in high histological grade, negative estrogen receptor and negative progesterone receptor of breast invasive ductal carcinoma tissues were higher(P < 0.05), and the nuclear expression rate in high histological grade tissue was significantly higher(P < 0.05).The tumor histological grade was an independent factor associated with high nuclear expression of HMGB1 in breast invasive ductal carcinoma(OR=2.197, P < 0.05).The tumor histological grade(OR=3.028, P < 0.01), estrogen receptor expression(OR=0.133, P < 0.01), and TNM stage(OR=3.817, P < 0.05) were the independent correlated factors for cytoplasmic positive expression of HMGB1 in breast invasive ductal carcinoma tissues.One hundred and thirty-nine breast cancer patients were followed up for 66.5(18-75) months, the overall 5-year survival rate and 5-year relapse-free survival rate were 88.49%(123/139) and 77.70%(108/139), respectively.The 5-year survival rate, 5-year relapse-free survival rate and survival curve among different nuclear and cytoplasmic expression groups were analyzed using Kaplan-Meier method and Log-rank it indicated that high expression of HMGB1 nucleus affected the 5-year relapse-free survival rate(P < 0.05).

ConclusionsThe high expression of HMGB1 in cell nucleus and cytoplasm is closely related to a number of poor prognostic factors in patients with breast invasive ductal carcinoma, and the high expression in nucleus is of guiding significance in predicting postoperative recurrence of breast invasive ductal carcinoma.The positive expression in cytoplasm is related to tumor histological grade, estrogen receptor and TNM stage.