Effect of circ_0000515 targeting miR-1258 on the proliferation and apoptosis in the colon cancer cells

ObjectiveTo explore the molecular mechanism of circ_0000515 regulating the proliferation and apoptosis of colon cancer cells.

MethodsA total of 41 cases of colon cancer patients with cancer tissue and adjacent tissue samples were selected.Real-time fluorescent quantitative PCR was used to detect the expression of circ_0000515 and miR-1258.Colon cancer cells SW620 were divided into si-circ_0000515 group, si-NC group, miR-1258 group, miR-NC group, si-circ_0000515+anti-miR-NC group, si-circ_0000515+anti-miR-1258 group.Tetramethylazolium salt colorimetric assay(MTT) was used to detect the cell viability.Flow cytometry was used to detect apoptosis.The targeting relationship of circ_0000515 and miR-1258 was detected by dual-luciferase reporter assay.

ResultsCompared with the adjacent tissues, the expression of circ_0000515 in colon cancer tissues was increased, and the expression of miR-1258 was decreased(P < 0.01).After the inhibition of circ_0000515 expression or overexpression of miR-1258, the viability of SW620 cells was decreased, the apoptosis rate was increased(P < 0.01).Circ_0000515 targeted and regulated the expression of miR-1258.Interference with miR-1258 expression reversed the effect of inhibiting circ_0000515 expression on the proliferation and apoptosis of colon cancer SW620 cells.

ConclusionsInhibiting the expression of circ_0000515 by up-regulation of miR-1258 can inhibit the proliferation and promote the apoptosis in colon cancer cells.