Effects of miR-503-5p on the proliferation, migration, apoptosis and adhesion of vascular endothelial cells induced by IL-1β

ObjectiveTo explore the effects of miR-503-5p on the proliferation, migration, apoptosis and adhesion of human umbilical vein endothelial cells(HUVECs) induced by interleukin1β(IL-1β), and its molecular mechanism.

MethodsThe HUVECs were divided into the NC group, IL-1β group, IL-1β+anti-miR-con group and IL-1β+anti-miR-503-5p group.The proliferation, adhesion, apoptosis and migration ability of cells were detected using MTT assay, adhesion assay, flow cytometry and Transwell assay, respectively.The expression levels of intercellular adhesion molecule 1(ICAM-1) and vascular cell adhesion molecule 1(VCAM-1) were detected using Western blotting.

ResultsCompared with the NC group, the cell viability and migrated cell numbers of HUVECs significantly reduced, and the cell adhesion numbers, apoptosis rate, and ICAM-1 and VCAM-1 protein expression levels significantly increased in the IL-1β group(P < 0.01).Compared with the IL-1β+anti-miR-con group, the cell viability and migrated cell numbers of HUVECs significantly increased, and the cell adhesion numbers, apoptosis rate, and ICAM-1 and VCAM-1 protein expression levels significantly decreased in the IL-1β+anti-miR-503-5p group(P < 0.01).

ConclusionsInhibiting the miR-503-5p expression can promote the vascular endothelial cell proliferation and migration, and inhibit vascular adhesion and apoptosis, which may be related to the inhibition of ICAM-1 and VCAM-1 expression.