ObjectiveTo study the effect of angiotensin Ⅱ on the proliferation of hepatoma HepG2 cells and its mechanism.
MethodsCCK-8 method was used to determine the effects of different concentrations of angiotensin Ⅱ on the proliferation of HepG2 cells treated for 24 and 48 hours.The expression levels of extracellular signal regulated kinase(ERK) 1/2 and angiotensin Ⅱ type 1 receptor(AT1R) in HepG2 cells treated with angiotensin Ⅱ were measured by Western blotting.
ResultsThe absorbance values of HepG2 cells treated with 10-6 and 10-5 mol/L angiotensin Ⅱ for 24 hours were higher than those in control group(P < 0.05), which in 10-5 mol/L angiotensin Ⅱ group was higher than that in 10-8 mol/L angiotensin Ⅱ group(P < 0.05).The absorbance values of HepG2 cells treated with 10-8, 10-7, 10-6, 10-5 and 10-4 mol/L angiotensin Ⅱ for 48 hours were higher than those in control group(P < 0.05).The expression levels of AT1R protein in HepG2 cells treated with 10-7, 10-6, 10-5 and 10-4 mol/L angiotensin Ⅱ were higher than those in the control group(P < 0.05).Among the 10-8, 10-6, 10-5 and 10-4 mol/L angiotensin Ⅱ groups, the expression of AT1R protein in HepG2 cells increased with the increase of drug concentration(P < 0.01).The expression of ERK1/2 protein in HepG2 cells treated with 10-6 mol/L angiotensin Ⅱ for 5, 10, 20 and 30 minutes did not change significantly(P>0.05), but the expression of phosphorylated ERK1/2 protein treated for 5 and 10 minutes was higher than that for 0, 20 and 30 minutes(P < 0.05).
ConclusionsAngiotensin Ⅱ may be through upregulating AT1R protein expression and promoting ERK1/2 phosphorylation to promote the abnormal proliferation of hepatoma HepG2 cells.
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